LEO User Manual

Learn how to use LEO and read results.

1. Introduction

2. How To Test With LEO

3. How To Read Result Of Test

4. Sampling Procedure

5. Tips For Effective Testing

6. Guidelines To Obtain Max Accuracy

7. Assessing Your Test Results

Watch Demo Get LEO?
1

Remove swab from sealed package and dip in a fresh glass of water.

2

For dry foods (bread/crackers), add a seed sized sample (3 mg) in the vial. For moist/wet (soup, mashed potatoes ) foods swab your plate up to 5 times.

3

Place swab, sample or a combination in the vial. Let it set for 30 seconds, close the cap and shake the vial 5 times.

4

Add 3 drops slowly into the "S" well on the LEO Gluten Free Tester. Add the drops slowly and no more than 3 drops.

5

After 3 minutes, look for a dark line above the "E" on LEO for a 10 PPM or less result. If you do not see a dark line or you see a very faint line, do NOT eat.

6

It is very important to scan your test with the LeoMyFood App. This will help you better understand the line darkness, help with GF research, quality control & a future LEO Tested restaurant guide.

3. How To Read Test Results

The tester has a window that displays the result. If you see a dark line above the "E" of LEO then the sample is less than or equal to 10 PPM. Please note that the O line is to show that the test worked correctly and the L is for future enhancements.

LEO Line Contrast Scale

4. Sampling Procedure for LEO

LEO cannot guarantee the absence of gluten on your entire plate, it’s reporting on the sample points that you used for the test.
  • When testing dry foods (bread, crackers, pretzels etc.) pinch a sesame size sample (3mg) in the bottle.
  • All other foods - swab your food/plate up to 5 times.
  • If the food is a thick liquid, just use a slight touch to sample, don’t stir or put a big glob on the swab.
  • Consider where the gluten may be present such as grill marks, cuts or touching from a shared spatula.
  • Before opening your test kit, we suggest you wash your hands and then open the package.
  • Loosen the purple lid of the bottle slightly. It has been tightened to ensure no leakage occurs during shipping.
  • Lay the gluten sensor chip on a flat surface and do not open the swab until ready to test.

It is recommended to swab multiple spots across your plate but not more than 5 spots.

  • When testing dry foods (bread, crackers, pretzels etc.) pinch a sesame size sample (3mg) in the bottle.
  • All other foods - swab your food/plate up to 5 times.
  • Remove the swab from the package, dip it into a glass of water and rub 5 different areas of the food.
  • Uncap the bottle and put the swab in the solution.
  • Drop the 3mg sample in the bottle and if also using some swab tests spin it five times, let the swab set in solution for 30 seconds, and press it against the edges as you gently pull the swab out of the tube to extract gluten from the swab.
  • Cap the bottle and shake it five times.

  • Twist the small white cap on the bottle.
  • Squeeze 3 drops of solution slowly into the sample (S) well of the tester.
  • Results will be displayed in the testing window within 3 minutes. (See line contrast scale for details).
  • Wash hands when finished with testing.

5. Tips For Effective Testing

Do not let the swab touch anything except the food you are concerned about.
Sensitivity
The gluten sensor is very sensitive to airborne gluten, such as in a bakery or pizza kitchen.
Substitutes
If an item is ordered with a gluten substitute, it is recommended to test the item that was substituted and the most likely point where contact may have occurred (grill marks, cut marks, spatulas, etc.)
Sampling
When testing dry foods (bread, crackers, pretzels etc.) pinch and place a sesame size sample (3mg) in the bottle.
Clean Space
LEO is highly sensitive to gluten detection. Please ensure your hands and testing surfaces are clean before conducting the test. Also avoid sampling an excessive amount for testing.

7. Assessing Your Test Results

Results Don't Mean A Guarantee. LEO is intended to give you additional data and level of comfort when eating, but is not a guarantee, as it can only provide a result for the sample being tested. It is still your responsibility to clearly communicate your food needs to the waitstaff at restaurants or friends & family preparing gluten-free meals for you, purchase items that are clearly labeled as gluten-free, and advocate for yourself. The decision of whether to consume something or not is your decision.
  • Have you eaten there before?
  • Do you trust the staff there?
  • Ask if the frying oil is used for products that are breaded?
  • Are the hash browns cooked in the same area that pancakes are cooked?
  • Is it certified gluten-free?
  • Is it labeled as gluten-free?
  • Have you consumed this item before?
  • Check the brand’s website for information on their quality control measures.

Speak pleasantly when speaking with restaurants or packaged food companies to make sure they want to help you and don’t feel they are being accused of anything.

LEO should always be used in conjunction with clear advocacy for gluten-free options and your better judgment.

LEO is intended to give you additional data and level of comfort when eating but is not a guarantee, as it can only provide a result for the sample being tested. It is still your responsibility to clearly communicate your food needs to the waitstaff at restaurants or friends & family preparing gluten-free meals for you, purchase items that are clearly labeled as gluten-free, and advocate for yourself. The decision of whether to consume something or not is your decision.